Improved procedures for purification of the Bandeiraea simplicifolia I isolectins and Bandeiraea simplicifolia II lectin by affinity chromatography.

An -N-acetyl-D-glycosamine binding lectin from Bandeiraea simplicifolia seeds.

An cY-n-galactopyranosyl binding lectin (1) was recently isolated and characterized from the seed extracts ofBandeiraea simplicifolia. While pursuing further studies on this lectin, a second protein capable of interacting with p-azophenyl 2-acetamido-2deoxy-aand -p-n-glucopyranoside BSA’ conjugates was discovered. The new lectin, isolated from the same plant seed by affinity chromatography, sho...

Resolution of nucleotide sugars and oligosaccharides by lectin affinity chromatography.

Bandeiraea simplicifolia I (BS I) isolectins, immobilized on Sepharose beads, specifically retarded low molecular weight ligands containing terminal cu-o-galactopyranosyl and 2acetamido-2-deoxy-a-D-galactopyranosyl residues. A BS I lectin-Sepharose column has been used to perform very efficient separations of structurally homologous sugar nucleotides and oligosaccharides. For example, UDP-gluco...

An immunochemical study of the combining sites of the second lectin isolated from Bandeiraea simplicifolia (BS II).

An Immunochemical Study of the Combining Sites of the Second Lectin Isolated from <i>Bandeiraea simplicifolia</i> (BS II)

BS II lectin: a second hemagglutinin isolated from Bandeiraea simplicifolia seeds with affinity for type III polyagglutinable red cells.

BS II lectin, a second hemagglutinin isolated from Bandeiraea simplicifolia seeds, agglutinated the red cells from 11 patients manifesting the 'acquired B' antigen phenomenon. The results of serological studies indicate that this lectin has specificity for the type III polyagglutinable receptors rather than for the 'B-like' antigens present on 'acquired B' red cells. Two other blood samples whi...